Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994;22(1):17-20.
doi: 10.1007/BF00431543.

Automated image analysis DNA cytometry in testicular cancer

R A Bürger  1 , U Witzsch, H Engel, D Potratz, M Stöckle
Affiliations

Automated image analysis DNA cytometry in testicular cancer

R A Bürger et al. Urol Res. 1994.

Abstract

The value of automated DNA cytometry for differentiation of testis cancer was evaluated in 54 seminomas, 13 HCG-positive seminomas, and 48 embryonal carcinomas. Slices of paraffin embedded tissue were enzymatically digested and stained with Feulgen SITS after fixation on glass slides. Automated DNA cytometry was performed with a Modular Image Analysis Computer (MIAC). DNA histogram phenotpye and computed DNA indices were correlated with the different tumor types. The ratio of hypertriploid to hypotriploid increased from HCG-positive seminoma over embryonal carcinoma to seminoma. The following mathematical DNA indices were found to correlate with tumor type: mean ploidy, 2c deviation index, 5c exceeding rate, variation coefficient of the GO/1 fraction and DNA nucleus diameter correlation.

PubMed Disclaimer

References

    1. Anal Quant Cytol. 1984 Mar;6(1):1-8 - PubMed
    1. Cancer. 1991 Apr 1;67(7):1874-7 - PubMed
    1. Beitr Pathol. 1974;153(4):370-8 - PubMed
    1. Int J Androl. 1987 Feb;10(1):115-20 - PubMed
    1. Mayo Clin Proc. 1988 Feb;63(2):103-12 - PubMed

LinkOut - more resources