Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Mar;8(2):143-9.
doi: 10.1007/BF01703071.

Baculovirus expression of the nucleocapsid protein of a Puumala serotype Hantavirus

C Schuldt  1 , L Zöller, E K Bautz, G Darai
Affiliations

Baculovirus expression of the nucleocapsid protein of a Puumala serotype Hantavirus

C Schuldt et al. Virus Genes. 1994 Mar.

Abstract

Recombinant baculoviruses were generated harboring the entire coding region of the S segment cDNA of Hantavirus strain CG 18-20 that belongs to the Puumala serotype. The recombinant nucleocapsid protein was expressed in Sf9 cells and shown to be antigenically identical with the authentic viral nucleocapsid protein by means of immunoblot analysis. Acute-phase and convalescent sera from European HFRS patients recognized the recombinant nucleocapsid protein in Western blots and the recombinant Baculovirus in indirect immunofluorescence assays. Insect cells infected with the recombinant Baculoviruses proved to be a suitable noninfectious substitute for Hantavirus-infected Vero E6 cells as an antigen source for immunodiagnostic assays allowing the detection of antibodies in HFRS patients.

PubMed Disclaimer

References

    1. Am J Trop Med Hyg. 1989 Jul;41(1):102-8 - PubMed
    1. J Clin Microbiol. 1993 May;31(5):1194-9 - PubMed
    1. DNA. 1985 Apr;4(2):165-70 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Virology. 1990 Jan;174(1):79-86 - PubMed

Publication types

LinkOut - more resources