Genetic mapping of sporulation operons in Bacillus subtilis using a thermosensitive sporulation mutant
- PMID: 807553
- PMCID: PMC246166
- DOI: 10.1128/jb.122.3.1109-1116.1975
Genetic mapping of sporulation operons in Bacillus subtilis using a thermosensitive sporulation mutant
Abstract
A thermosensitive sporulation mutant was used to determine the order of sporulation operonsin the urs region of the Bacillus subtilis chromosome. Data from three-factor transformation crosses and three- and four-factor transduction crosses established the order metC-SPO-96(SpoII)-spo-85(SpoV)-spo-279(SpoII)-furA-ura-cysC-spo-NG1.67(SpoIII). Previously, furA was thought to lie to the right of ura and cysC to the left (Dubnau, 1970; Young and Wilson, 1972).
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