Human erythrocyte rosette formation with mitogen-stimulated human lymphocytes--a marker for the demonstration of activated T-cells

Abstract

Human lymphocytes after stimulation by various mitogens could be shown to make rosettes with washed uncoated human Group O rhesus negative erythrocytes (H-rosettes). Using purified T- and B-cell populations and exposing them to the action of mitogens it has been demonstrated that the cell responsible is the activated T-lymphocyte. The H-rosette is thus presented as a new marker for the demonstration of activated human T-cells.