doi: 10.1073/pnas.91.18.8462.
Binding of a soluble alpha beta T-cell receptor to superantigen/major histocompatibility complex ligands
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- PMID: 8078904
- PMCID: PMC44626
- DOI: 10.1073/pnas.91.18.8462
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Binding of a soluble alpha beta T-cell receptor to superantigen/major histocompatibility complex ligands
Proc Natl Acad Sci U S A.
.
Abstract
The genes for the alpha and beta chains of a murine T-cell receptor were truncated just prior to the portions encoding the transmembrane regions and introduced into baculovirus by recombination. Insect cells infected with the virus secreted a soluble form of the receptor that could be purified to homogeneity. This soluble receptor reacted with a set of six monoclonal antibodies originally raised to different epitopes on the natural transmembrane-region-containing receptor and bound with appropriate specificity to a cell surface complex of the human major histocompatibility complex class II molecule DR1 with the bacterial superantigen staphylococcal enterotoxin B.
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