Futher investigations of the gamma-chaina in a ghanaian adult, homozygous for hereditary perisitence of fetal haemoglobin. Isolation of gamma CB-3 peptides and Ggamma:Agamma ratio determination in human Hb E

Abstract

The gamma-chain in a Ghanain homozygous for hereditary persistence of fetal haemoglobin was considered to be of the Ggamma type on the basis of the amino acid analysis of gammaTp XIV (gamma133-144) of the Hb F of this subject [1]. Recently, the sequence of residues gamma134-137 of the gamma-chain of this subject was determined and found to contain some alanine at position gamma136. It is therefore of the Ggamma + Agamma type. A rapid technique for the isolation of gammaCB-3 (gamma134-146) peptides in human fetal haemoglobin for Ggamma:Agamma ratio determination is described.