Availability of monovalent and divalent cations within intact chloroplasts for the action of ionophores nigericin and A23187

Abstract

1. A23187 will uncouple electron transport by broken chloroplasts in a divalent cation dependent manner provided that they have been treated with a low concentration of EDTA. 2. A23187 stimulates oxaloacetate-dependent oxygen evolution and inhibits phosphoglycerate reduction by intact chloroplasts isolated in a cation-free medium whereas the full effect of nigericin was dependent on the presence of external K+. 3. Uncoupling of oxaloacetate reduction by A23187 in intact chloroplasts is inhibited by EDTA and this effect is overcome by excess Mg2+. 4. The results suggest that divalent and not monovalent cations are available for collapsing the light-induced H+ gradient within the intact organelle.