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. 1975 Feb 17;280(7):877-80.

[Immunocytochemical localization of hyaluronidase in the spermatozoa of domestic mammals]

[Article in French]
J E Fléchon, M P Dubois
  • PMID: 809170

[Immunocytochemical localization of hyaluronidase in the spermatozoa of domestic mammals]

[Article in French]
J E Fléchon et al. C R Acad Hebd Seances Acad Sci D. .

Abstract

Smears of washed spermatozoa are treated by an indirect immunocytochemical technique. The first antiserum used is prepared in rabbits against ovine (or bovine) hyaluronidase. A sheep antiserum against rabbit globulin, labeled with fluorescerine or peroxidase, is used at the second reagent. Hyaluronidase is localized in the anterior segment of the sperm acrosomes of ram, bull and other species. The specificity of the immunocytochemical staining is checked by appropriate controls. Anti-hyaluronidase serum adsorbed with the antigen, or normal serum, is used as the first reagent. The spermatozoa are also treated with the labeled antiserum only.

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