doi: 10.1073/pnas.72.7.2559.
3'-Terminal nucletide sequence (n equals 361) of bacteriophage MS2 RNA
- PMID: 809766
- PMCID: PMC432808
- DOI: 10.1073/pnas.72.7.2559
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3'-Terminal nucletide sequence (n equals 361) of bacteriophage MS2 RNA
Proc Natl Acad Sci U S A.
1975 Jul.
Abstract
32P-Labeled MS2 RNA was partially digested with ribonuclease T1 (guanyloribonuclease; ribonucleate 3'-guanylo-oligonucleotidohydrolase; EC 3.1.4.8) or with epilson-carboxymethyl-lysine-41 pancreatic ribonuclease A (ribonucleate 3'-pyrimidino-oligonucleotidohydrolase; EC 3.1.4.22). A series of overlapping fragments was obtained which allowed the reconstruction of a 361-nucleotide-long 3'-terminal sequence. A unique reading frame could be deduced, which indicated that the replicase gene ends with a U-A-G termination signal and is followed by a 174-nucleotide-long untranslated segment.
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