Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Jul;72(7):2559-62.
doi: 10.1073/pnas.72.7.2559.

3'-Terminal nucletide sequence (n equals 361) of bacteriophage MS2 RNA

A Vandenberghe, W Min Jou, W Fiers

3'-Terminal nucletide sequence (n equals 361) of bacteriophage MS2 RNA

A Vandenberghe et al. Proc Natl Acad Sci U S A. 1975 Jul.

Abstract

32P-Labeled MS2 RNA was partially digested with ribonuclease T1 (guanyloribonuclease; ribonucleate 3'-guanylo-oligonucleotidohydrolase; EC 3.1.4.8) or with epilson-carboxymethyl-lysine-41 pancreatic ribonuclease A (ribonucleate 3'-pyrimidino-oligonucleotidohydrolase; EC 3.1.4.22). A series of overlapping fragments was obtained which allowed the reconstruction of a 361-nucleotide-long 3'-terminal sequence. A unique reading frame could be deduced, which indicated that the replicase gene ends with a U-A-G termination signal and is followed by a 174-nucleotide-long untranslated segment.

PubMed Disclaimer

References

    1. Nature. 1970 Jul 18;227(5255):271-3 - PubMed
    1. J Mol Biol. 1972 Nov 14;71(2):243-61 - PubMed
    1. Eur J Biochem. 1974 Jan 16;41(2):413-20 - PubMed
    1. Nature. 1972 May 12;237(5350):82-8 - PubMed
    1. J Mol Biol. 1967 Dec 28;30(3):507-27 - PubMed

Associated data