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. 1993 Jun 7;324(1):81-6.
doi: 10.1016/0014-5793(93)81537-a.

Characterisation of a chimeric hD3/D2 dopamine receptor expressed in CHO cells

G McAllister  1 , M R Knowles, S Patel, R Marwood, F Emms, G R Seabrook, M Graziano, D Borkowski, P J Hey, S B Freedman
Affiliations
Free article

Characterisation of a chimeric hD3/D2 dopamine receptor expressed in CHO cells

G McAllister et al. FEBS Lett. .
Free article

Abstract

The D2 dopamine receptor is known to be functionally coupled when expressed in CHO cells, whereas the effector systems for the D3 dopamine receptor remain unclear. A chimeric, human D3/D2 receptor (hD3/D2) was constructed containing the third intracellular loop region of the D2 receptor. CHO cells stably expressing the D2, D3, or hD3/D2 receptors were created and the pharmacology of the receptors was examined. The chimeric hD3/D2 receptor retained D3-like affinities for dopaminergic ligands. However, in contrast to the D2 receptor neither the D3 receptor nor the hD3/D2 receptor could functionally couple to the adenylate cyclase or arachidonic acid release mechanisms.

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