[Detection of expression of a membrane form of the guanylate cyclase type of GC-B in cattle retina]

Abstract

cDNA clones encoding the central and C-terminal parts of a membrane-bound guanylate cyclase (GC) were isolated from the lambda ZAP bovine retinal library. All of the analysed recombinants appeared to carry inserts encoding the guanylate cyclase GC-B. Analysis of the determined nucleotide and deduced amino acid sequences showed extremely high level of homology to the sequences of known GC-B. The results indicate that a mRNA for GC-B is expressed in the bovine retina.