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. 1975;137(4):289-304.
doi: 10.1007/BF00703255.

Regulated in vitro synthesis of the enzymes of the deo operon of Escerichia coli. properties of the DNA directed system

B A Svenningsen

Regulated in vitro synthesis of the enzymes of the deo operon of Escerichia coli. properties of the DNA directed system

B A Svenningsen. Mol Gen Genet. 1975.

Abstract

The four enzymes deoxyriboaldolase, thymidine phosporylase, deoxyribomutase, and purine nucleoside phosphorylase have been synthesized in substantial amounts in a DNA-dependent in vitro system programmed with DNA containing the deo operon. The synthesis is greatly stimulated by deoxyribose-5-phosphate and cyclic AMP indicating that the deoR repressor and the catabolite activating protein (CAP) are highly active under our cell-free conditions. In contrast it has not yet been possible to observe a reproducible effect of the cytR repressor in vitro. The sequential appearance of active enzymes has confirmed the direction of transcription as being dra-tpp-drm-pup and has indicated that the four genes are transcribed into a single tetracistronic message.

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