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Comparative Study
. 1994 Feb 11;139(1):123-5.
doi: 10.1016/0378-1119(94)90534-7.

Cloning and sequencing of the Leishmania major actin-encoding gene

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Comparative Study

Cloning and sequencing of the Leishmania major actin-encoding gene

M V de Arruda et al. Gene. .

Abstract

We report the cloning and characterization of the genomic sequence of the actin (Act)-encoding gene (act) from Leishmania major. Restriction maps of two genomic clones, as well as genomic Southern analysis strongly suggest that the act of L. major is a single-copy gene. A single 1.6-kb transcript is detected in Northern blots. The deduced amino-acid sequence shows 68-89% identity with Act sequences from other eukaryotes.

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