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. 1975 Nov;12(5):1189-94.
doi: 10.1128/iai.12.5.1189-1194.1975.

Use of Bacillus subtilis minicells to demonstrate an antigenic relationship between the poles and lateral cylindrical regions of rod-shaped cells

S I Coyne, N H Mendelson

Use of Bacillus subtilis minicells to demonstrate an antigenic relationship between the poles and lateral cylindrical regions of rod-shaped cells

S I Coyne et al. Infect Immun. 1975 Nov.

Abstract

Purified minicells produced by Bacillus subtilis div IV-B1 mutants were used to immunize rabbits. Immune serum was obtained that agglutinated minicells and was able to form precipitin lines when reacted with minicell antigens in double-diffusion or immunoelectrophoretic procedures. Antiminicell serum agglutinated rod-shaped B. subtilis cells to long filaments produced by growth of a cell division-defective mutant at restrictive temperature. These findings indicate that minicells are immunogens capable of eliciting the production of antibodies that cross-react with the lateral, cylindrical regions of B. subtilis rods. It appears, therefore, that poles share a common antigen(s) with cylindrical regions of the cell.

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References

    1. J Bacteriol. 1972 Mar;109(3):1266-72 - PubMed
    1. J Bacteriol. 1974 Mar;117(3):1330-4 - PubMed
    1. J Bacteriol. 1974 Mar;117(3):1312-9 - PubMed
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