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. 1994 Mar;69(3):463-7.
doi: 10.1038/bjc.1994.84.

Inhibition of leukaemia cell proliferation by folic acid-polylysine-mediated introduction of c-myb antisense oligodeoxynucleotides into HL-60 cells

G Citro  1 , C Szczylik, P Ginobbi, G Zupi, B Calabretta
Affiliations
Free PMC article

Inhibition of leukaemia cell proliferation by folic acid-polylysine-mediated introduction of c-myb antisense oligodeoxynucleotides into HL-60 cells

G Citro et al. Br J Cancer. 1994 Mar.
Free PMC article

Abstract

The inhibitory effect of c-myb antisense oligodeoxynucleotides (ODNs) conjugated to folic acid (FA) on HL-60 cell proliferation was examined. Folic acid was covalently linked to a polylysine chain and purified by gel chromatography. Sterile FA-polylysine was complexed with c-myb sense and antisense. Exposure of HL-60 cells to the FA-polylysine-c-myb antisense ODN complex resulted in a down-regulation of c-myb expression and a greater inhibition of proliferation than that obtained using free ODNs. Moreover, FA-polylysine conjugate alone or complexed to c-myb sense ODN was not toxic to cells. The antigenic properties and uptake of the vitamin were not affected by the polylysine chain. These data suggest that this strategy is potentially useful for the selective delivery of anti-oncogene-targeted ODNs into cancer cells.

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References

    1. Biochim Biophys Acta. 1975 May 16;390(3):298-311 - PubMed
    1. Science. 1993 Aug 20;261(5124):1004-12 - PubMed
    1. J Biol Chem. 1984 May 25;259(10):6601-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Aug;83(16):5983-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9636-40 - PubMed

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