Affinity chromatographic purification of the pregnancy zone protein
- PMID: 81241
- DOI: 10.1016/0022-1759(78)90115-1
Affinity chromatographic purification of the pregnancy zone protein
Abstract
An immunospecific affinity chromatographic method for purification of human pregnancy zone protein (PZP) directly from serum is described. Highly purified goat-anti-human PZP-immunoglobulin was applied as a ligand. Recovery of PZP varied from 56--75%. The impurities constituted maximally 5--10% of the total protein in the eluate. The purification factor was approximately 100.
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