Comparative Study
doi: 10.1002/elps.11501401174.
Human erythrocyte pyrimidine 5'-nucleotidase isoenzymes: effect of sulfhydryl reagents and electrophoretic discrimination
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- PMID: 8125061
- DOI: 10.1002/elps.11501401174
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Comparative Study
Human erythrocyte pyrimidine 5'-nucleotidase isoenzymes: effect of sulfhydryl reagents and electrophoretic discrimination
Electrophoresis.
1993 Oct.
Abstract
The electrophoretic behavior of human red cell pyrimidine 5'-nucleotidase isozymes (UMPH1 and UMPH2) was studied on starch gels with and without treatment with thiol reagents. It was found that at least one reactive sulfhydryl group occurs in the UMPH1 isozyme but not in the UMPH2 isozyme. An electrophoretic system is described that allows the discrimination of UMPH1 and UMPH2 isozymes.
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