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. 1994 Mar 11;140(1):121-6.
doi: 10.1016/0378-1119(94)90741-2.

Cloning and sequence determination of the gene encoding sorbitol dehydrogenase from Saccharomyces cerevisiae

A V Sarthy  1 , C Schopp, K B Idler
Affiliations

Cloning and sequence determination of the gene encoding sorbitol dehydrogenase from Saccharomyces cerevisiae

A V Sarthy et al. Gene. .

Abstract

The identification of a sorbitol-induced sorbitol dehydrogenase (SDH) activity from Saccharomyces cerevisiae is described. The SDH1 structural gene was isolated from a lambda gt11 yeast genomic library using an antibody to a 40-kDa protein induced in yeast cells growing in medium containing sorbitol. The gene encodes a 357-amino-acid (aa) protein deduced from the nucleotide sequence. Comparison of the aa sequence of the yeast SDH1 with that of sheep liver SDH reveals a 63% overall similarity. Yeast transformants containing the cloned gene carried on a multicopy plasmid express high levels of SDH1 only when grown on sorbitol, suggesting that the cloned gene contains both regulatory and coding sequences.

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