Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Feb 25;22(4):639-45.
doi: 10.1093/nar/22.4.639.

On the rapid deprotection of synthetic oligonucleotides and analogs

N N Polushin  1 , A M Morocho, B C Chen, J S Cohen
Affiliations
Free PMC article

On the rapid deprotection of synthetic oligonucleotides and analogs

N N Polushin et al. Nucleic Acids Res. .
Free PMC article

Abstract

The efficiency of oligodeoxynucleotide deprotection is greatly enhanced using a combination of: (a) ethanolamine, and especially a mixture of hydrazine, ethanolamine and methanol, in place of the usual aqueous ammonia; (b) tert-butylphenoxyacetyl amino protecting groups, and (c) oxalyl link between the first nucleotide and the polymeric support. The extent of base modification, particularly of C, is shown to be extremely low, and the quality of deprotected oligonucleotides is as high as in the case of ammonia deprotection. This method is also shown to be applicable to the preparation of phosphorothioate and methylphosphonate oligodeoxynucleotides and oligoribonucleotides.

PubMed Disclaimer

References

    1. Biochemistry. 1986 Sep 9;25(18):5092-7 - PubMed
    1. Nucleic Acids Res. 1987 Jan 26;15(2):397-416 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Aug;85(16):5764-8 - PubMed
    1. Nucleic Acids Res. 1989 May 11;17(9):3501-17 - PubMed
    1. Nucleic Acids Res. 1989 Jun 26;17(12):4863-71 - PubMed

Publication types

MeSH terms