Developmental expression of acid-base-related proteins in the rabbit kidney

Abstract

The newborn is limited in its ability to respond to acid-base perturbations. To investigate the development of renal H+/HCO3- transport mechanisms, we probed acid-base-related epitopes in the mesonephric and developing metanephric kidneys of rabbits. Using immunofluorescence with monoclonal antibodies to the vacuolar H+ATPase, band 3-like Cl-/HCO3- exchanger, and apical surface of fully differentiated beta-intercalated cells, and peanut lectin cytochemistry (another marker of beta-intercalated cells), we found that these epitopes were poorly expressed in the nephrogenic zone of the newborn kidney cortex. Deeper in the cortex, collecting ducts showed weak apical staining with beta-intercalated cell antibodies and two patterns of staining with the H+ATPase and band 3 antibodies: polar and circumferential or diffuse. Some cells showed apical staining with H+ATPase while others showed diffuse staining, similar to that observed in the mature cortical collecting duct. Band 3 labeling was basolateral, as observed in the adult, and diffuse, which was rarely seen in mature kidney sections. Newborn outer medullary collecting ducts showed apical labeling with H+ATPase and basolateral staining with band 3 antibodies, similar to the mature outer medulla. Surprisingly, the mesonephric collecting tubule showed cells with apical H+ATPase staining or basolateral band 3 labeling and, less frequently, cells with positive staining for beta-intercalated cells. The relative maturity of the mesonephric collecting tubule and similarity to what is observed in mature metanephric collecting ducts indicates that intercalated cells may be present and functioning in both organs. Thus, the lineage of intercalated cells may be more intricate than previously believed.