LR Gold and LR White embedding of lung tissue for immunoelectron microscopy

Abstract

The immunogold technique in conjunction with LR Gold or LR White embedding was used to localize different antigens in normal rat and mini pig lung tissues. Lycopersicon esculentum and Maclura pomifera lectins and diverse cytoskeletal antigens such as cytokeratins, beta-tubulin, smooth muscle-actin, tropomyosin and villin were labelled well. Factor VIII-related antigen was found in Weibel-Palade bodies of endothelial cells. A 10 kDa Clara cell protein was localized in Clara cells. The morphological preservation of LR Gold-embedded tissue was superior to that of LR White-embedded tissues, but significant differences in antigenicity were not seen.