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. 1975 Nov 25;197(2):145-52.
doi: 10.1007/BF02388780.

Electron microscopic localization of carbonic anhydrase (CA) activity in rabbit cornea

S Yokota, W K Waller

Electron microscopic localization of carbonic anhydrase (CA) activity in rabbit cornea

S Yokota et al. Albrecht Von Graefes Arch Klin Exp Ophthalmol. .

Abstract

The localization of CA activity in the rabbit cornea was investigated using a modified Hansson's method. The reaction products indicating CA activity was observed in the lateral membrane of endothelial cell, but not in the apical membrane. In cytoplasm, Golgi complex, and mitochondria of the endothelial cells no specific reaction product could be found. In epithelium, stroma, and Descemet's membrane, no reaction product was seen. The specific staining was inhibited in the control group which was treated with 10(-4)M sodium acetazolamide.

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