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. 1994 Feb;60(2):746-8.
doi: 10.1128/aem.60.2.746-748.1994.

Recognition of chimeric small-subunit ribosomal DNAs composed of genes from uncultivated microorganisms

E D Kopczynski  1 , M M Bateson, D M Ward
Collaborators, Affiliations

Recognition of chimeric small-subunit ribosomal DNAs composed of genes from uncultivated microorganisms

E D Kopczynski et al. Appl Environ Microbiol. 1994 Feb.

Abstract

When PCR was used to recover small-subunit (SSU) rRNA genes from a hot spring cyanobacterial mat community, chimeric SSU rRNA sequences which exhibited little or no secondary structural abnormality were recovered. They were revealed as chimeras of SSU rRNA genes of uncultivated species through separate phylogenetic analysis of short sequence domains.

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