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. 1994 Mar 21;341(2-3):277-80.
doi: 10.1016/0014-5793(94)80472-9.

Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein

S Inouye  1 , F I Tsuji
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Free article

Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein

S Inouye et al. FEBS Lett. .
Free article

Abstract

Expression of the cDNA for Aequorea green fluorescent protein in E. coli yielded a fused protein with fluorescence excitation and emission spectra virtually identical to those of the native green fluorescent protein. Further, a solution of the protein, when mixed with aequorin and calcium ion, emitted a greenish luminescence characteristic of the in vivo luminescence of the animal, indicating a radiationless energy transfer to the protein.

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