Improved enzymatic hydrolysis of hair
- PMID: 8138218
- DOI: 10.1016/0379-0738(93)90271-b
Improved enzymatic hydrolysis of hair
Abstract
An enzymatic hair extraction method is proposed for drug analysis. Pronase digestion of various aliquots of hair from a cocaine abuser was preceded by a 2-h incubation with a dithiothreitol solution. The extraction solution was tested to identify possible interferences in the radioimmunoassay and was compared with other hydrolysis methods to assess the results of extraction.
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