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. 1994 Mar 15;298 Pt 3(Pt 3):619-22.
doi: 10.1042/bj2980619.

Production of human embryonic haemoglobin (Gower II) in a yeast expression system

R M Mould  1 , O M Hofmann, T Brittain
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Production of human embryonic haemoglobin (Gower II) in a yeast expression system

R M Mould et al. Biochem J. .

Abstract

The cDNA coding for a human embryonic globin protein has been obtained from an erythroleukaemic cell line. A plasmid expression system for human embryonic haemoglobin Gower II containing cDNA copies of the appropriate pair of globin genes coupled to synthetic galactose-regulated hybrid promoters has been engineered. Transformation of Saccharomyces cerevisiae with this plasmid yields a cellular system capable of high-level production of fully functional tetrameric embryonic haemoglobin. We have developed a purification scheme which gives high yields of pure human embryonic haemoglobin suitable for structural and functional studies. Preliminary characterization studies are reported.

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References

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