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. 1994 Jan;3(1):70-81.
doi: 10.1002/pro.5560030110.

Solution structure of villin 14T, a domain conserved among actin-severing proteins

M A Markus  1 , T Nakayama, P Matsudaira, G Wagner
Affiliations

Solution structure of villin 14T, a domain conserved among actin-severing proteins

M A Markus et al. Protein Sci. 1994 Jan.

Abstract

The solution structure of the N-terminal domain of the actin-severing protein villin has been determined by multidimensional heteronuclear resonance spectroscopy. Villin is a member of a family of actin-severing proteins that regulate the organization of actin in the eukaryotic cytoskeleton. Members of this family are built from 3 or 6 homologous repeats of a structural domain of approximately 130 amino acids that is unrelated to any previously known structure. The N-terminal domain of villin (14T) contains a central beta-sheet with 4 antiparallel strands and a fifth parallel strand at one edge. This sheet is sandwiched between 2 helices on one side and a 2-stranded parallel beta-sheet with another helix on the other side. The strongly conserved sequence characteristic of the protein family corresponds to internal hydrophobic residues. Calcium titration experiments suggest that there are 2 binding sites for Ca2+, a stronger site near the N-terminal end of the longest helix, with a Kd of 1.8 +/- 0.4 mM, and a weaker site near the C-terminal end of the same helix, with a Kd of 11 +/- 2 mM. Mutational and biochemical studies of this domain in several members of the family suggest that the actin monomer binding site is near the parallel strand at the edge of the central beta-sheet.

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References

    1. Protein Sci. 1992 Jun;1(6):736-51 - PubMed
    1. Adv Protein Chem. 1981;34:167-339 - PubMed
    1. Cell. 1988 Jul 15;54(2):139-40 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Jul;85(14):4986-90 - PubMed
    1. J Biol Chem. 1988 Nov 15;263(32):16738-43 - PubMed

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