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Comparative Study
. 1994 Mar 29;91(7):2752-6.
doi: 10.1073/pnas.91.7.2752.

Molecular cloning and functional expression of two monocyte chemoattractant protein 1 receptors reveals alternative splicing of the carboxyl-terminal tails

I F Charo  1 , S J Myers, A Herman, C Franci, A J Connolly, S R Coughlin
Affiliations
Comparative Study

Molecular cloning and functional expression of two monocyte chemoattractant protein 1 receptors reveals alternative splicing of the carboxyl-terminal tails

I F Charo et al. Proc Natl Acad Sci U S A. .

Abstract

Monocyte chemoattractant protein 1 (MCP-1) is a member of the chemokine family of cytokines that mediate leukocyte chemotaxis. The potent and specific activation of monocytes by MCP-1 may mediate the monocytic infiltration of tissues in atherosclerosis and other inflammatory diseases. We have isolated cDNAs that encode two MCP-1-specific receptors with alternatively spliced carboxyl tails. Expression of the receptors in Xenopus oocytes conferred robust mobilization of intracellular calcium in response to nanomolar concentrations of MCP-1 but not to related chemokines. The MCP-1 receptors are most closely related to the receptor for the chemokines macrophage inflammatory protein 1 alpha and RANTES (regulated on activation, normal T expressed and secreted). The identification of the MCP-1 receptor and cloning of two distinct isoforms provide powerful tools for understanding the specificity and signaling mechanisms of this important chemokine.

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References

    1. Nucleic Acids Res. 1987 Oct 26;15(20):8125-48 - PubMed
    1. Anal Biochem. 1987 Apr;162(1):156-9 - PubMed
    1. Biochem Biophys Res Commun. 1989 Feb 28;159(1):249-55 - PubMed
    1. J Exp Med. 1989 Apr 1;169(4):1449-59 - PubMed
    1. FASEB J. 1989 Dec;3(14):2565-73 - PubMed

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