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. 1978 Sep;75(9):4257-61.
doi: 10.1073/pnas.75.9.4257.

3'-terminal nucleotide sequence of encephalomyocarditis virus RNA determined by reverse transcriptase and chain-terminating inhibitors

D Zimmern, P Kaesberg

3'-terminal nucleotide sequence of encephalomyocarditis virus RNA determined by reverse transcriptase and chain-terminating inhibitors

D Zimmern et al. Proc Natl Acad Sci U S A. 1978 Sep.

Abstract

We have adapted the chain-termination method for determining the nucleotide sequence of DNA of Sanger, Nicklen, and Coulson [(1977) Proc. Natl. Acad. Sci. USA 74, 5463--5467] for use with reverse transcriptase (RNA-directed DNA nucleotidyltransferase) on RNA templates. With this method and using a primer (the octanucleotide pdT7rC) directed at the 3'-terminal poly(A), we have determined a sequence of 166 residues in the genomic RNA of the picornavirus encephalomyocarditis virus.

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References

    1. J Mol Biol. 1975 May 25;94(3):441-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. Nucleic Acids Res. 1977 Aug;4(8):2527-38 - PubMed
    1. Biochemistry. 1974 Jul 16;13(15):3159-63 - PubMed
    1. J Gen Virol. 1970 Mar;6(3):373-80 - PubMed

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