Restriction endonuclease analysis and molecular cloning of porcine adenovirus type 3

Abstract

The 6618 strain of porcine adenovirus type 3 was cultivated in swine testis cells, and viral DNA was extracted from the infected cells by a modified Hirt procedure. The DNA was digested by each of 13 restriction endonucleases, and the number of cleavage sites which were identified varied from 1 to 17. The size of the porcine adenovirus type 3 genome, estimated from the sizes of the restriction enzyme fragments, was approximately 35 kb. Fragments representing the entire genome were cloned. Physical maps were constructed based on the results of restriction endonuclease analyses and hybridization and oriented with respect to the genome of bovine adenovirus type 3.