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. 1994 Mar;169(3):683-5.
doi: 10.1093/infdis/169.3.683.

Use of DNA moderately repetitive sequence to type Aspergillus fumigatus isolates from aspergilloma patients

H Girardin  1 , J Sarfati, H Kobayashi, J P Bouchara, J P Latgé
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Use of DNA moderately repetitive sequence to type Aspergillus fumigatus isolates from aspergilloma patients

H Girardin et al. J Infect Dis. 1994 Mar.

Abstract

Polymorphism of forty-seven sequential clinical isolates from 3 patients with an aspergilloma was analyzed. DNA from each isolate was digested with EcoRI and hybridized in Southern blots with a 32P-labeled nonribosomal DNA repetitive sequence. Most isolates from each patient displayed the same hybridization pattern. Southern blot patterns obtained with DNA repetitive sequences can be used to type clinical isolates of Aspergillus fumigatus and have shown that aspergilloma patients are most probably infected by a single strain.

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