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. 1994 Apr 12;91(8):2905-8.
doi: 10.1073/pnas.91.8.2905.

Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement

C L Peterson  1 , A Dingwall, M P Scott
Affiliations

Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement

C L Peterson et al. Proc Natl Acad Sci U S A. .

Abstract

The Saccharomyces cerevisiae SWI1, SWI2 (SNF2), SWI3, SNF5, and SNF6 gene products play a crucial role in the regulation of transcription. We provide here direct biochemical evidence that all five SWI/SNF polypeptides are components of a large multisubunit complex. These five polypeptides coelute from a gel-filtration column with an apparent molecular mass of approximately 2 MDa. The five SWI/SNF polypeptides do not copurify when extracts are prepared from swi- or snf- mutants. We show that SWI/SNF polypeptides also remain associated during an affinity-chromatography step followed by gel filtration. Assembly of the SWI/SNF complex is not disrupted by a mutation in the putative APT-binding site of SWI2, although this mutation eliminates SWI2 function.

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Comment in

  • Dissecting a complex process.
    LaMarco K. LaMarco K. Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):2886-7. doi: 10.1073/pnas.91.8.2886. Proc Natl Acad Sci U S A. 1994. PMID: 8159673 Free PMC article. Review. No abstract available.

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