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. 1994 Mar;60(3):1014-7.
doi: 10.1128/aem.60.3.1014-1017.1994.

A method to detect enteroviruses in sewage sludge-amended soil using the PCR

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A method to detect enteroviruses in sewage sludge-amended soil using the PCR

T M Straub et al. Appl Environ Microbiol. 1994 Mar.

Abstract

PCR detection of seeded poliovirus type 1 in sludge-amended soil was made possible by utilizing Sephadex G-50 and Chelex-100 resins to remove compounds present in sludge-amended soil that may inhibit PCR. With this method, enteroviruses indigenous to an anerobically digested sludge were detected by PCR in 10 different soils amended with this sludge.

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