A method to detect enteroviruses in sewage sludge-amended soil using the PCR
- PMID: 8161168
- PMCID: PMC201425
- DOI: 10.1128/aem.60.3.1014-1017.1994
A method to detect enteroviruses in sewage sludge-amended soil using the PCR
Abstract
PCR detection of seeded poliovirus type 1 in sludge-amended soil was made possible by utilizing Sephadex G-50 and Chelex-100 resins to remove compounds present in sludge-amended soil that may inhibit PCR. With this method, enteroviruses indigenous to an anerobically digested sludge were detected by PCR in 10 different soils amended with this sludge.
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