The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: in vitro and in vivo studies

Abstract

Following in vitro screening investigations on approximately 100 nematophagous fungi reported previously, eight species were selected for further investigation. Fungal elements (mycelium and conidia) were subjected to in vitro stress selection designed to simulate rumen and abomasal conditions. From these studies, three species, namely, Arthrobotrys oligospora, Arthrobotrys oviformis and Geniculifera eudermata, were selected for in vivo survival studies in sheep surgically fitted with abomasal and ileal cannulae. Doses of fungal conidia were administered orally or via the abomasal cannulae and samples of digesta were taken from the abomasum, the terminal ileum and faeces. The viability of the three fungal species at these sites was demonstrated. The abundance of fungi throughout the gut was dose-dependent but in all cases only very small volumes of fungal suspension containing unprotected conidia were used. These results demonstrate that a practical means of orally administering nematophagous fungi to control free-living stages of nematodes in faeces may become an achievable objective.