Human monoclonal antibody Fab fragments cloned from combinatorial libraries: potential usefulness in prevention and/or treatment of major human viral diseases
- PMID: 8173784
Human monoclonal antibody Fab fragments cloned from combinatorial libraries: potential usefulness in prevention and/or treatment of major human viral diseases
Abstract
Currently, there is increased interest in the use of human viral antibodies for prophylaxis and therapy because recent advances in molecular immunology have made it possible to generate large numbers of human monoclonal antibodies of desired specificity and functional activity in short order. The strategy, developed particularly at The Scripps Research Institute and at Cambridge University is based on antigen selection of such antibodies from combinatorial libraries that express Fabs on the surface of filamentous DNA bacteriophage. Fabs or their whole immunoglobulin derivatives that exhibit high avidity for the selecting antigen and high neutralizing activity for the corresponding virus have been identified, and many of these human monoclonal antibodies should prove to be useful in prophylaxis or therapy of presently uncontrolled, medically important human viral diseases.
Similar articles
-
Recombinant human respiratory syncytial virus (RSV) monoclonal antibody Fab is effective therapeutically when introduced directly into the lungs of RSV-infected mice.Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1386-90. doi: 10.1073/pnas.91.4.1386. Proc Natl Acad Sci U S A. 1994. PMID: 8108420 Free PMC article.
-
Human Fab antibodies derived from phage display library neutralize hepatitis A virus in vitro.Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2000 Dec;14(4):313-6. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2000. PMID: 11471014
-
Bacterial expression of a human recombinant monoclonal antibody fab fragment against hepatitis B surface antigen.J Med Virol. 1999 Aug;58(4):338-45. J Med Virol. 1999. PMID: 10421399
-
Cloning allergen-specific antibody fragments (Fabs); tools for allergen standardization and therapy of type I allergy.Arb Paul Ehrlich Inst Bundesamt Sera Impfstoffe Frankf A M. 1997;(91):222-9. Arb Paul Ehrlich Inst Bundesamt Sera Impfstoffe Frankf A M. 1997. PMID: 9383913 Review.
-
Recombinant antibodies.Hum Antibodies Hybridomas. 1991 Oct;2(4):172-89. Hum Antibodies Hybridomas. 1991. PMID: 1751781 Review.
Cited by
-
A recombinant single chain antibody neutralizes coronavirus infectivity but only slightly delays lethal infection of mice.Eur J Immunol. 1997 Dec;27(12):3447-55. doi: 10.1002/eji.1830271245. Eur J Immunol. 1997. PMID: 9464834 Free PMC article.
-
Characterization of a type-common human recombinant monoclonal antibody to herpes simplex virus with high therapeutic potential.J Clin Microbiol. 1998 Nov;36(11):3198-204. doi: 10.1128/JCM.36.11.3198-3204.1998. J Clin Microbiol. 1998. PMID: 9774565 Free PMC article.
-
High mutant frequency in populations of a DNA virus allows evasion from antibody therapy in an immunodeficient host.J Virol. 2003 Feb;77(4):2701-8. doi: 10.1128/jvi.77.4.2701-2708.2003. J Virol. 2003. PMID: 12552010 Free PMC article.
-
Production and characterization of a human recombinant monoclonal Fab fragment specific for influenza A viruses.Clin Diagn Lab Immunol. 2003 Jul;10(4):680-5. doi: 10.1128/cdli.10.4.680-685.2003. Clin Diagn Lab Immunol. 2003. PMID: 12853404 Free PMC article.
-
Passive immunotherapy for retroviral disease: influence of major histocompatibility complex type and T-cell responsiveness.Proc Natl Acad Sci U S A. 1995 Nov 7;92(23):10492-5. doi: 10.1073/pnas.92.23.10492. Proc Natl Acad Sci U S A. 1995. PMID: 7479826 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Medical