Antigens of pig follicular fluid and ovarian tissue and the effect of anti-ovarian serum on pig oocytes and spermatozoa

Abstract

Common antigens were detected in pig follicular tissue, blood serum, blood plasma, seminal plasma and kidney, liver, testicular and ovarian tissue. If pig oocytes were exposed to the action of anti-ovarian antibodies, no precipitation of the zona pellucida was observed. In immunofluorescence, however, reaction of the zona, which was unaffected by absorption of this antiserum by any of the above antigens, was found. Antisera against pig spermatozoa, seminal plasma, follicular fluid, and testicular tissue did not react with the antigens on oocytes. Similarly, anti-ovarian and antifollicular sera had no effect on boar spermatozoa.