Differentiation of Purkinje cells and their relationship to other components of developing cerebellar cortex in man

Abstract

The differentiation of Purkinje cells and their relationship to other components of the developing cerebellar cortex were analyzed by the Golgi impregnation method and by electron microscopy in human specimens of various pre- and postnatal ages. The three stages of Purkinje cell maturation that have been previously recognized in other species are also evident in man: the first stage occupies primarily the fourth fetal month (12-16 weeks); the second stage lasts through the fifth, sixth and seventh feta months (16-28 weeks); the third stage extends throughout the remaining period of intrauterine life and the first postnatal year and continues at a slow rate thereafter. During the first stage, Purkinje cells are distributed in a layer, several rows deep. Their bipolar somas are relatively smooth and have only a few processes at the apical and basal cell poles. In the 3-month period of the second stage, Purkinje cells become gradually organized into a single row. Their somas become invested with additional randomly oriented dendritic processes and numerous somatic spines (pseudopodia). The first morphologically well-defined synapses appear on the Purkinje cell somatic spines and on their immature dendritic shafts at the beginning of the second stage and become more prominent during the period from 18 to 24 weeks. In the third stage, the dendritic arbor becomes flattened in the plane transverse to the folium and somatic spines disappear. Spines appear on the secondary and tertiary dendrites between the twenty-fourth and twenty-eighth fetal weeks and continue to increase in number during the entire third stage as new dendritic branches develop. These observations indicate that cellular maturation and synaptogenesis in the primate cerebellum differ from these events in non-primate species, with respect to time of birth, in the relative duration of each phase and in the total time necessary for neuronal differentiation. The protracted time of differentiation and the slow growth of Purkinje cell dendrites in man may be due to the numerically complex relationships existing between granule and Purkinje cells. It is probably not simply a reflection of the larger size of human Purkinje cells and their dendrites.