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. 1994 May 9;344(1):65-8.
doi: 10.1016/0014-5793(94)00371-8.

Immunological detection of prohormone convertases in two different proglucagon processing cell lines

P Blache  1 , D Le-Nguyen, C Boegner-Lemoine, A Cohen-Solal, D Bataille, A Kervran
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Free article

Immunological detection of prohormone convertases in two different proglucagon processing cell lines

P Blache et al. FEBS Lett. .
Free article

Abstract

The distribution of the prohormone convertases, PC1/3, PC2 and PC5/6, was determined by immunoblotting in two cell lines. In alpha TC1-6 cells, the proglucagon processing occurred according to the pancreatic A-cell type. In STC-1 cells, proglucagon was processed in a manner reminiscent of the intestinal L-cell type. PC1/3 was undetectable in both proglucagon processing cell lines whereas PC2 displayed a strong immunostaining in the alpha TC1-6 cells and was barely detectable in the STC-1 cells. PC5/6 was detected as a 70 kDa protein in both cell lines. These results suggest a possible role of PC2 in the processing of proglucagon into glucagon in the A-cells, whereas in L-cells it would require still undetermined endoproteases.

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