[Creation of transgenic plants Nicotiana tabacum and Solanum tuberosum, resistant to the herbicide phosphinothricin]

Abstract

The expression cassette for phosphinothricin acetyltransferase gene (PAT or bar) from S. hygroscopicus has been constructed on the basis of the pBI121 plasmid. Leaf disks of N. tabacum cv. SR1 and steam segments of S. tuberosum cv. Prigozhii-2 have been transformed using Agrobacterium. Selection of the transformed plants carried out by PCR analysis revealed 12 transgenic tobacco plants and 3 transgenic potato plants. bar gene expression and plant resistance to phosphinothricin treatment have been studied in regenerated plants. All the transgenic plants were found to be resistant to the herbicide. Mendelian inheritance of the inserted gene has been proved by analysis of the F1 progeny of transformed tobacco plants.