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. 1994 May 16;200(3):1735-41.
doi: 10.1006/bbrc.1994.1653.

Production of human PTH(1-34) via a recombinant DNA technique

S Nakagawa  1 , Y Tamakashi, Y Ishibashi, M Kawase, S Taketomi, O Nishimura, T Fukuda
Affiliations

Production of human PTH(1-34) via a recombinant DNA technique

S Nakagawa et al. Biochem Biophys Res Commun. .

Abstract

The production of hPTH(1-34) by site specific chemical cleavage of [Cys35]hPTH(1-84) obtained by a biotechnology technique is described. The amino-peptide bond of S-cyanylated cysteine residues in peptides or proteins is specifically cleaved by exposure to an alkaline solution (Stark, G. R. (1977) Methods Enzymol, 47, 129-132). However, when applying this method to [Cys35]hPTH(1-84), we observed many by-products. Formation of these by-products was suppressed using a short reaction in 0.03N NaOH containing 6M urea at low temperature, and hPTH(1-34) was specifically produced. The product was indistinguishable from standard hPTH(1-34) with respect to chemical and biological characterization.

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