Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Dec;38(4):371-80.
doi: 10.1007/BF01907983.

Genomic cloning and partial characterization of human chymotrypsinogen gene

D X Hou  1 , K Ozawa, N Tomita, Y Maeda, T Hashiguchi, K Yokoyama, E Soeda
Affiliations

Genomic cloning and partial characterization of human chymotrypsinogen gene

D X Hou et al. Jpn J Hum Genet. 1993 Dec.

Abstract

Chymotrypsinogen is a principal precursor of pancreatic proteolytic enzymes. We previously isolated a cDNA clone for human prechymotrypsinogen from a human pancreatic cDNA library. In the present study, we used this cDNA sequences to isolate genomic DNA clones. Three overlapping cosmid clones spanning approximately 65-kb genomic sequences were isolated from a human cosmid library. The genomic DNA clones were characterized by restriction enzyme mapping and by hybridizing them to subfragments of the cDNA. The sequence tagged sites for human chymotrypsinogen gene were created by designing two oligonucleotides. Furthermore, the isolated genomic clones were confirmed to be localized on chromosome 16q23 by fluorescence in situ hybridization and G-banding analysis.

PubMed Disclaimer

References

    1. Somat Cell Mol Genet. 1984 Jul;10(4):369-76 - PubMed
    1. Biochim Biophys Acta. 1975 Feb 27;379(2):431-43 - PubMed
    1. Genomics. 1987 Dec;1(4):313-9 - PubMed
    1. J Biol Chem. 1984 Nov 25;259(22):14265-70 - PubMed
    1. Science. 1990 Jan 5;247(4938):64-9 - PubMed

Publication types

LinkOut - more resources