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Comparative Study
. 1994 May;176(10):2962-9.
doi: 10.1128/jb.176.10.2962-2969.1994.

Systematic study of the 3-hydroxy fatty acid composition of mycobacteria

S Alugupalli  1 , F Portaels, L Larsson
Affiliations
Comparative Study

Systematic study of the 3-hydroxy fatty acid composition of mycobacteria

S Alugupalli et al. J Bacteriol. 1994 May.

Abstract

Twenty-seven strains belonging to 12 Mycobacterium species were studied for 3-hydroxy fatty acid composition. Mycobacterial cells were subjected to both mild and strong acid methanolysis, after which the liberated hydroxy fatty acids were purified and analyzed by gas chromatography-mass spectrometry as methyl ester trimethylsilyl ether derivatives. Altogether, 21 3-hydroxy fatty acids containing 14 to 28 carbon atoms were detected; 10 were straight chain, 6 were 2-methyl branched chain, and 5 were 2,4,6-trimethyl branched chain. The mycobacterial strains were classified in groups according to 3-hydroxy fatty acid patterns.

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