Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1994 Mar;24(5):825-30.
doi: 10.1007/BF00029865.

Molecular cloning of mannose-binding lectins from Clivia miniata

E J Van Damme  1 , K Smeets, F Van Leuven, W J Peumans
Affiliations
Comparative Study

Molecular cloning of mannose-binding lectins from Clivia miniata

E J Van Damme et al. Plant Mol Biol. 1994 Mar.

Abstract

Screening of a cDNA library constructed from total RNA isolated from young developing ovaries of Clivia miniata Regel with the amaryllis lectin cDNA clone resulted in the isolation of four different isolectin clones which clearly differ from each other in their nucleotide sequences and hence also in their deduced amino acid sequences. Apparently the lectin is translated from an mRNA of ca. 800 nucleotides encoding a precursor polypeptide of 163 amino acids. Northern blot analysis of total RNA isolated from different tissues of Clivia miniata has shown that the lectin is expressed in most plant tissues with very high lectin mRNA concentrations in the ovary and the seed endosperm.

PubMed Disclaimer

References

    1. Anal Biochem. 1990 Nov 15;191(1):25-30 - PubMed
    1. Digestion. 1990;46 Suppl 2:308-16 - PubMed
    1. Nucleic Acids Res. 1986 Jun 11;14(11):4683-90 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. Eur J Biochem. 1992 Jun 1;206(2):413-20 - PubMed

Publication types

LinkOut - more resources