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. 1976;11(3-4):273-9.
doi: 10.1016/0022-1759(76)90120-4.

Isolation of human T and B lymphocytes by rosette formation with 2-aminoethylisothiquronium bromide (AET) -treated sheep red blood cells with monkey red blood cells

M A Pellegrino, S Ferrone, A N Theofilopoulos

Isolation of human T and B lymphocytes by rosette formation with 2-aminoethylisothiquronium bromide (AET) -treated sheep red blood cells with monkey red blood cells

M A Pellegrino et al. J Immunol Methods. 1976.

Abstract

Purified human B and T lymphocytes were obtained by rosetting HPL with AET-SRBC or MRBC and separating the non-rosetted from the rosetted cells on Ficoll-Hypaque gradient. 92 +/- 5% of the B cells were fluorescent positive for mBlg and 95 +/- 2% of the purified T cells rosetted with AET-SRBC. 69 +/- 5% of the B cells and 61 +/- 8% of the T cells present in the unifractionated HPL were recovered in the purified fractions.

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