Properties of Drosophila simulans strains experimentally infected by different clones of the bacterium Wolbachia

Abstract

Maternally inherited bacteria of the genus Wolbachia are responsible for reproductive incompatibilities between strains of Drosophila simulans. Such incompatibilities are known in several types of crosses, including both directions of crossing between two types of infected strains, 'R' and 'S'. To determine whether the bidirectional incompatibility between R and S flies is due only to differences between their bacteria, flies from an uninfected strain have been experimentally infected with bacteria associated with each type. The incompatibility properties of experimental strains are close to those of original strains harbouring the same bacteria and therefore independent of nuclear background. Backcross experiments, however, show that the infection level of a strain depends on the nature of paternal ancestors. This is not explained by nuclear effects but is possibly the result of an interaction between the infection levels of both parents, in which the infection level of S strains is an equilibrium between a tendency for females to produce weakly infected offspring and selection of more infected eggs by sperm from infected males.