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. 1994 Apr;85(4):357-63.
doi: 10.1111/j.1349-7006.1994.tb02367.x.

Correlation between tumorigenicity and expression levels or splicing patterns of transcripts of the human papillomavirus type 16 E6 gene

T Inoue  1 , S Kyo, T Kiyono, M Ishibashi, H Ishiwatari, Y I Hwang, M Yutsudo, A Hakura
Affiliations

Correlation between tumorigenicity and expression levels or splicing patterns of transcripts of the human papillomavirus type 16 E6 gene

T Inoue et al. Jpn J Cancer Res. 1994 Apr.

Abstract

For determination of the correlation between tumorigenicity and the expression levels or splicing patterns of E6 mRNAs of the human papillomavirus type 16 in established cells, a vector containing the intact E6 open reading frame which expresses both unspliced and spliced mRNAs, one expressing only unspliced E6 mRNA, and one expressing both unspliced and spliced mRNAs but producing only truncated E6 proteins were constructed. In transformation assays and analyses of E6 mRNAs, a higher expression level of unspliced E6 mRNA was found to be closely associated with tumorigenicity. Furthermore, it was also related with anchorage-independent growth and a decreased serum requirement of the cells.

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References

    1. ) Gissman , L. , Wolnik , L. , Ikenberg , H. , Koldvsky , U. , Schnurch , G. and zur Hausen , H.Human papillomavirus types 6 and 11 DNA sequences in genital and laryngeal papillomas and in some cervical biopsies . Proc. Natl. Acad. Sci. USA , 80 , 560 – 563 ( 1983. ). - PMC - PubMed
    1. ) zur Hausen , H.Human papillomaviruses in the pathogenesis of anogenital cancer . Virology , 184 , 9 – 13 ( 1991. ). - PubMed
    1. ) Shimada , M. , Fukushima , M. , Mukai , H. , Kato , I. , Nishikawa , A. and Fujinaga , K.Amplification and specific detection of transforming gene region of human papillomavirus 16, 18 and 33 in cervical carcinoma by means of the polymerase chain reaction . Jpn. J. Cancer Res. , 81 , 1 – 5 ( 1990. ). - PMC - PubMed
    1. ) Sasagawa , T. , Inoue , M. , Tanizawa , O. , Yutsudo , M. and Hakura , A.Identification of antibodies against human papillomavirus type 16 E6 and E7 proteins in sera of patients with cervical neoplasias . Jpn. J. Cancer Res. , 83 , 705 – 713 ( 1992. ). - PMC - PubMed
    1. ) Hawley‐Nelson , P. , Vousden , K. H. , Hubbert , N. L. , Lowy , D. R. and Schiller , J. T.HPV I6 E6 and E7 proteins cooperate to immortalize primary human foreskin keratinocytes . EMBO J. , 8 , 3905 – 3910 ( 1989. ). - PMC - PubMed

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