Cytoskeletal organization of the vestibular supporting cells. Saponin perfusion method for observing intracellular structures by scanning electron microscopy

Abstract

The cytoskeletal organization of the guinea pig vestibular supporting cells was investigated employing the saponin perfusion method using scanning electron microscopy. The skeletal framework of a cell was composed of actin, intermediate filaments and microtubules. The membrane bound organelles such as nucleus mitochondria, endoplasmic reticulum, Golgi apparatus, etc. were also well documented. This made it possible to investigate the three-dimensional organization of cytoskeletons as well as their complex interaction with various membrane bound organelles. The intermediate filaments were usually seen surrounding the nucleus and extending through the cytoplasm which connected the nuclear membrane to the plasma membrane, reticular lamina or other cytoorganelles. The intermediate filaments also closely related to the desmosomes. These findings suggest that the intermediate filaments provide mechanical support to the cell and its nucleus. The microtubules were closely related to the secretory granules, Golgi apparatus and intermediate filaments, which supports the idea that the microtubules may control the distribution of intermediate filaments, and also play an important role for the transport of the secretory granules.