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. 1994 Jan;8(1):47-54.
doi: 10.1007/BF01703601.

A preliminary translational map of the frog virus 3 genome

P D Foglesong  1 , D B Willis
Affiliations

A preliminary translational map of the frog virus 3 genome

P D Foglesong et al. Virus Genes. 1994 Jan.

Abstract

A preliminary map of the frog virus 3 (FV 3) genome was constructed by hybridization-selection of mRNAs to cloned DNA fragments and translation in reticulocyte lysates. FV 3 mRNAs were hybridized to KpnI, HindIII, and SalI restriction fragments representing the entire FV 3 genome. Two different hybridization conditions were employed in order to discriminate between the hybridization of early and late mRNAs. A total of 43 major and 18 minor early genes and nine major and three minor late genes were mapped. A 24 kb region comprised of the KpnI D, H, and E fragments encodes 8 of the 12 late genes that were mapped.

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