doi: 10.1016/0923-2508(93)90043-2.
Presence of mrr- and mcr-like restriction systems in coryneform bacteria
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- PMID: 8210675
- DOI: 10.1016/0923-2508(93)90043-2
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Presence of mrr- and mcr-like restriction systems in coryneform bacteria
Res Microbiol.
1993 Mar-Apr.
Free article
Abstract
Efficient transformation of Brevibacterium flavum MJ233C and Corynebacterium glutamicum ATCC 31831 (up to 5.0 x 10(7) transformants/microgram DNA) depends on the source of plasmid DNA. The transformation efficiencies of B. flavum MJ233C and C. glutamicum ATCC 31831 increased nearly 10(3)-fold when plasmid DNA was isolated from the recipient strain itself or from a damdcm Escherichia coli mutant, as compared with DNA passed through a modification-proficient E. coli strain. These results suggest the presence of a methyl-specific restriction system in certain strains of coryneform bacteria. In addition, electroporation conditions were optimized.
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