Comparison of the immunogenicity and antigenic composition of ten Central American snake venoms
- PMID: 8212043
- DOI: 10.1016/0041-0101(93)90263-i
Comparison of the immunogenicity and antigenic composition of ten Central American snake venoms
Abstract
The immunological reactivity of five crotaline antivenoms for the venoms of ten Costa Rican snakes was determined. Venoms from Bothrops asper, B. godmani, B. lateralis, B. nasutus, B. ophryomegas, B. schlegelii, B. nummifer, B. picadoi, Crotalus durissus durissus and Lachesis muta stenophrys were separated by SDS-PAGE, transferred to cellulose nitrate membrane and reacted against five different antivenoms. Antisera used in the immunoblotting were prepared in rabbits to the crotaline venoms from Crotalus viridis viridis (prairie rattlesnake), Crotalus durissus terrificus (South American rattlesnake), Crotalus atrox (western diamondback rattlesnake), and Bothrops atrox (fer de lance). SDS-PAGE analysis of the ten venoms indicated that all venoms had components in the high-medium mol. wt (> 15,000) and low mol. wt (< 15,000) range, but they all had at least twice as many components in the high-medium mol. wt range. The venoms of B. nummifer and B. nasutus have the greatest number of bands (24) whereas B. asper has the lowest (17). There appeared to be no difference in immunogenicity between high-medium mol. wt components and low mol. wt components; however, with the venoms of B. nasutus, B. ophryomegas, and B. schlegelii, there were few reactions between antivenoms and low mol. wt components. Half of the ten venoms tested had the highest reactivity with antivenom against B. atrox venom. Two venoms reacted most with antivenom against C. adamanteus venom; one with antivenom to C. atrox venom; one with antivenom to C. v. viridis venom and one with antivenom to C. d. terrificus venom.(ABSTRACT TRUNCATED AT 250 WORDS)
Similar articles
-
Comparison of the immunogenicity and antigenic composition of several venoms of snakes in the family Crotalidae.Toxicon. 1990;28(2):189-99. doi: 10.1016/0041-0101(90)90413-2. Toxicon. 1990. PMID: 2339435
-
Contributions of the snake venoms of Bothrops asper, Crotalus simus and Lachesis stenophrys to the paraspecificity of the Central American polyspecific antivenom (PoliVal-ICP).Toxicon. 2018 Mar 15;144:1-6. doi: 10.1016/j.toxicon.2018.01.016. Epub 2018 Feb 4. Toxicon. 2018. PMID: 29407870
-
Cross-reactivity and cross-immunomodulation between venoms of the snakes Bothrops asper, Crotalus simus and Lachesis stenophrys, and its effect in the production of polyspecific antivenom for Central America.Toxicon. 2017 Nov;138:43-48. doi: 10.1016/j.toxicon.2017.08.009. Epub 2017 Aug 9. Toxicon. 2017. PMID: 28803057
-
Immunological profile of antivenoms: preclinical analysis of the efficacy of a polyspecific antivenom through antivenomics and neutralization assays.J Proteomics. 2014 Jun 13;105:340-50. doi: 10.1016/j.jprot.2014.02.021. Epub 2014 Feb 28. J Proteomics. 2014. PMID: 24583507 Review.
-
Production of monovalent anti-Bothrops asper antivenom: development of immune response in horses and neutralizing ability.Rev Biol Trop. 1988 Nov;36(2B):511-7. Rev Biol Trop. 1988. PMID: 3078800 Review.
Cited by
-
Bothrops atrox, the most important snake involved in human envenomings in the amazon: How venomics contributes to the knowledge of snake biology and clinical toxinology.Toxicon X. 2020 Apr 23;6:100037. doi: 10.1016/j.toxcx.2020.100037. eCollection 2020 Jun. Toxicon X. 2020. PMID: 32550592 Free PMC article.
-
Development of sandwich ELISA and lateral flow assay for the detection of Bungarus multicinctus venom.PLoS Negl Trop Dis. 2023 Mar 30;17(3):e0011165. doi: 10.1371/journal.pntd.0011165. eCollection 2023 Mar. PLoS Negl Trop Dis. 2023. PMID: 36996245 Free PMC article.